Protein_Domain
AmCyan

Part:BBa_J176022:Design

Designed by: Karmella Haynes   Group: Haynes Lab   (2011-09-29)

AmCyan


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • PCR-cloned from pAmCyan1-C1 (Clontech)
  • Primers add a XbaI site upstream and SpeI/NotI/PstI downstream:
    • Forward 5'-CCTTTCTAGAatggccctgtccaac
    • Reverse 5'-AAGGCTGCAGCGGCCGCTACTAGTgaagggcaccacgga
  • Mutated at position 510 to remove a PstI site (site directed mutagenesis primer 5'-gatgctgcaAggcggcggca)


Source

  • pAmCyan1-C1 (Clontech)


References

  • Matz, M. V., et al. (1999) Nature Biotech. 17:969–973